瑞典Medicago成立于1995年,是一家通過了ISO9001認證的高科技生物制品企業(yè),致力于設計、研發(fā)和生產方便使用的標準包裝的生物試劑、診斷試劑盒及生化試劑盒。公司產品已被*廣大科研人員采用;所提供的產品包含smart緩沖液及緩沖試劑(大范圍高純度的預混SmartBuffers與SmartReagents ,便于使用的包裝片劑或粉劑。該系列產品在常規(guī)實驗中確保溶液高度穩(wěn)定的前提下可節(jié)省大量的科研時間,增加實驗結果的準確性)、超純外源凝集素(作為世界*的凝集素研發(fā)和生產供應商之一,Medicago采用親和色譜法純化出高品質和超高純度的凝集素產品)、皂苷類(Medicago是為數不多的展業(yè)研發(fā)和生產皂苷類產品的生物高科技企業(yè),可為只要企業(yè)及診斷試劑盒生產客戶提供不同包裝規(guī)格的皂苷類產品)、生物活性蛋白(Medicago的生物活性蛋白產品包括高品質的鈣結合蛋白、鈣調蛋白及其他實驗室常用蛋白產品,憑借其豐富的蛋白研發(fā)優(yōu)勢, Medicago供應的生物活性蛋白不僅具有高純度,還具有超高的生物活性)及免疫/診斷試劑/生物醫(yī)藥檢測試劑盒(在免疫診斷領域,Medicago提供高品質的單/多克隆抗體產品,以及純化自病毒、細菌及寄生蟲中的抗原產品。其研發(fā)生產的基于well-proven ELISA及單克隆抗體技術的診斷試劑盒產品廣受用戶的青睞)產品五大系列,其高品質低價格的系列產品為*用戶廣泛采用,部分產品同時由Sigma和Fluka公司OEM銷售。其生產的片裝PBS更是深受*用戶的青睞!除了上述上述即買的產品外,Medicago還根據客戶的特殊需要提供定制或貼牌生產服務,規(guī)格與包裝可任選,以滿足不同客戶的多種需要。
Triticum vulgaris lectin (WGA)
Article No | Product name | Pack Size |
---|---|---|
05-0102-1000 | Absolute Lectins | 1000 mg |
05-0102-10 | WGA | 10 mg |
05-0102-25 | WGA | 25 mg |
05-0102-100 | WGA | 100 mg |
特征
產品描述
Triticum vulgaris lectin or Weat germ agglutinin (WGA) is isolated from Triticum vulgaris (Weat germ) and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 36 kDa. WGA selectively binds to N-Acetyl glucosamine (GlcNAc) and to N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids (1).
The WGA lectin agglutinates erythrocytes and most types of malignant cells. It agglutinates rabbit erythrocytes at < 0.1 µg/ml following trypsin treatment of the cells. Adding 300 mM N-Acetyle-D-Glucosamine gives an inhibition with a titer that is at least 8-fold lower than the control.
Wheat germ agglutinin inhibits the C receptor interaction, which has implications in studies of receptor micro-heterogeneity and ligand binding sites (1).
WGA together with Con A is the lectin most widely used as an analytical and preparative agent when studying glycoproteins and cell surface proteins. The immobilized lectin can be used for affinity chromatography of cells and sub-cellular particles.
Medicago’s WGA is supplied as a white to pale yellow lyophilized powder from a buffer containing 10 mM CH3COONH4. No preservatives are added. The purity of WGA is determined by SDS- electrophoresis which generates three bands, corresponding to the three isolectins of WGA. Protein content is verified in spectrophotmetry The lectin is available in vials containing 100 mg, 25 mg or 10 mg powder and the product is to be used for laboratory work only.
應用
使用指南
凝膠素可以在使用前用2ml去離子水重構。輕輕旋轉小瓶,直至*溶解。認為在高濃度的2-巰基乙醇的存在下發(fā)生聚集。
運輸和存儲
該產品的運輸時間為-20°C,但對于日常運輸,可能會在環(huán)境溫度下運輸。冷凍干燥粉從生產之日起至少保持在-20℃以下穩(wěn)定三年以上。用去離子水重構后,溶液可以冷凍保存在等分試樣中長達12個月。
參考
(1) Johnson R. J., Simpson S., Van Epps D. E., Chenoweth D. E. (1992) Wheat germ agglutinin inhibits the C receptor interaction: implications for receptor microheterogeneity and ligand binding site. Journal of Leukocyte Biology Vol 52, Issue 1, 3–10.
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