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Kapa Biosystems 產(chǎn)品報(bào)價(jià) 2016

發(fā)布時(shí)間：2016/4/27      點(diǎn)擊次數(shù)：5160

Kapa是世界上的生物技術(shù)公司?？偛课挥诿绹ㄊ款D, 公司的主要理念是運(yùn)用分子和遺傳學(xué)技術(shù)來打造新一代的生物科學(xué)和醫(yī)學(xué)診斷產(chǎn)品。
Kapa公司目前主要是在“直接進(jìn)化（directed evolution technology platform）”的技術(shù)平臺(tái)上來篩選和生產(chǎn)高品質(zhì)的生物酶。目前生物醫(yī)藥領(lǐng)域所使用的大部分生物酶都只是天然存在形式，即使有些酶經(jīng)過基因工程改造，但是由于酶分子自身的結(jié)構(gòu)和功能限制，要想得到真正滿足科研工作人員需要的產(chǎn)品，卻是一件費(fèi)時(shí)費(fèi)力的事情，而且常常達(dá)不到預(yù)期的要求。“直接進(jìn)化”技術(shù)正是針對這一現(xiàn)實(shí)情況，運(yùn)用遺傳，基因工程，生物信息學(xué)等技術(shù)來在分子水平上設(shè)計(jì)真正滿足不同實(shí)際要求的生物酶。通過創(chuàng)新，Kapa為廣大的科研人員提供了一系列擁有多項(xiàng)技術(shù)的產(chǎn)品，不僅能夠滿足普通科研需要，同時(shí)為高通量，基因芯片等的普及創(chuàng)造了條件。 運(yùn)用“直接進(jìn)化”技術(shù)，Kapa目前已經(jīng)開發(fā)出來了以下的高品質(zhì)產(chǎn)品： 
   1. Kapa2G*PCR （Kapa2G Robust PCR）。適用范圍：可用于普通的PCR反應(yīng)中；AT和GC堿基含量高的擴(kuò)增；模板 中含有常見的PCR反應(yīng)抑制物，例如，鹽離子，尿素，SDS,乙醇，EDTA等；石蠟油包被樣品；糞便樣品；菌落PCR。 
   2. Kapa2G 快速PCR（Kapa2G Fast PCR）。高靈敏度，高特異性。延伸速度高達(dá)1秒/kb，（常規(guī)延伸速度是60s/kb），可擴(kuò)增出長達(dá)5kb的目的片段，節(jié)省大約75%的反應(yīng)時(shí)間。 
   3.實(shí)時(shí)熒光定量PCR （Kapa SYBR qPCR）。和其他公司的類似產(chǎn)品相比，Kapa SYBR qPCR改造了Taq DNA多聚酶，而不是對熒光染料進(jìn)行化學(xué)改造，因此大大提高了信號強(qiáng)度以及反應(yīng)的有效性。更重要的是，Kapa的系列產(chǎn)品可以適用于市場上現(xiàn)有的Real-time PCR儀器。 
   4.Kapa長片段DNA多聚酶 （Kapa LongRange）。Kapa長片段DNA多聚酶是由Taq DNA聚合酶和修飾過的archael（B型）高保真DNA聚合酶組成的雙酶系統(tǒng)。后者含有校正功能。這種雙酶系統(tǒng)是專門為長片段和敏感PCR擴(kuò)增而設(shè)計(jì)的。一般而言，普通的Taq酶由于缺少校正功能，因此隨著錯(cuò)誤堿基的不斷產(chǎn)生，使得擴(kuò)增過早的終止，從而大大降低了擴(kuò)增效率。這也是導(dǎo)致在擴(kuò)增長片段或者模板濃度很低時(shí)，使用普通Taq酶常常失敗的原因。另一方面，雖然具有校正能力的多聚酶擁有很高的正確率，但由于其3到5的外切酶活性，引物會(huì)被降解掉，從而也影響到擴(kuò)增的靈敏度。Kapa長片段產(chǎn)品的原理是：在Taq DNA多聚酶中加入具有校正能力并且修飾過的多聚酶，可以使得不匹配的堿基被修正；同時(shí)，和只有校正功能的多聚酶相比，kapa的雙酶系統(tǒng)中具有校正功能的酶所占的比例較少，因此可以相對延長擴(kuò)增的循環(huán)數(shù)和時(shí)間，從而提高產(chǎn)量，同時(shí)得到高保真的PCR產(chǎn)物。雖然兩種酶都有5到3的DNA聚合功能，但是只有Taq酶具有依賴于雙鏈的5到3的聚合酶活性,而只有B型DNA聚合酶具有3到5的外切酶功能（也就是校正功能）。 
   5.Kapa高保真DNA多聚酶 （Kapa Fidelity）。是經(jīng)過修飾的，耐熱的高保真DNA多聚酶。既有5到3的多聚酶活性，又有3到5外切校正功能，因此能夠擴(kuò)增出的高保真片段。高保真：Kapa高保真DNA多聚酶的擴(kuò)增出正確片段的幾率是普通Taq酶的15倍,KOD/Pfx2倍；應(yīng)用廣泛：可以擴(kuò)增出長至6kb的質(zhì)?；蛘遧ambda DNA,或者長至2kb的基因組DNA擴(kuò)增；用量少：由于其高活性，和其它的高保真產(chǎn)品相比，每個(gè)反應(yīng)的用量要少，從而為實(shí)驗(yàn)者節(jié)省資金；易于克?。篕apa高保真DNA多聚酶擴(kuò)增出鈍頭產(chǎn)物，非常易于克??；快速：擴(kuò)增速度是pfu的2-3倍。 另外，還有普通的KapaTaq DNA多聚酶，以及熱啟動(dòng)酶,T4連接酶，快速連接試劑盒等產(chǎn)品。

 

部分產(chǎn)品報(bào)價(jià):

第二代基因工程酶及相關(guān)產(chǎn)品
產(chǎn)品歸類廠家貨號品名規(guī)格目錄價(jià)
HiFi高保真酶KAPAKK2101HiFi100 units700
KAPAKK2102HiFi250 units1600
KAPAKK2501HiFi Hot Start100 units800
KAPAKK2502HiFi Hot Start250 units1800
KAPAKK2601HiFi Hot Start100 rxn800
KAPAKK2602HiFi Hot Start500 rxn3500
HiFi高保真酶（二代測序）KAPAKK2611HiFi Hot Start for NGS50 rxn800
KAPAKK2612HiFi Hot Start for NGS250 rxn3500
KAPAKK2701HiFi Hot Start for NGS50 rxn2400
KAPAKK2702HiFi Hot Start for NGS250 rxn10000
KAPAKK2801KAPA HiFi  HS Uracil +ready Mix50 x 50µL3000
KAPAKK2802KAPA HiFi  HS Uracil +ready Mix250 x 50µL 14000
長片段酶KAPAKK3006Long Range100 units800
KAPAKK3005Long Range250 units1600
KAPAKK3501Long Range Hot Start100 units900
KAPAKK3502Long Range Hot Start250 units1800
KAPAKK3503Long Range Hot Start500 units3000
KAPAKK3601Long Range Hot StartReady Mix with DYE  100 x 25µL1100
KAPAKK3602Long Range Hot StartReady Mix with DYE  500 x 25µL4500
與takara酶相對應(yīng)的KAPA Extaq酶KAPAKK3009KAPATaq Extra（針對takara ExTaq）250units400
KAPAKK3505KAPATaq Extra Hot Start（針對takara ExTaq）250units520
KAPAKK3604KAPATaq Extra Hot Start Ready Mix with DYE （針對takara ExTaq） 100 x 25µL400
KAPAKK3605KAPATaq Extra Hot StartReady Mix with DYE（針對takara ExTaq） 500 x 25µL 1800
KAPAKK3606KAPATaq Extra Hot Start Ready Mix with DYE 200 x 25µL800
HRMKAPAKK4201KAPA HRM FAST1ml700
KAPAKK4202KAPA HRM FAST5ml3200
KAPAKK4203KAPA HRM FAST10ml6000
SYBR熒光定量KAPAKK4600SYBR® Fast通用型1 ml400
KAPAKK4601SYBR® Fast通用型5 ml1800
KAPAKK4602SYBR® Fast通用型10 ml3500
KAPAKK4618SYBR® Fast通用型50 ml17000
KAPAKK4603SYBR® Fast(ABI Prism）1 ml400
KAPAKK4604SYBR® Fast(ABI Prism）5 ml1800
KAPAKK4605SYBR® Fast(ABI Prism）10 ml3500
KAPAKK4617SYBR® Fast(ABI Prism）50ml17000
KAPAKK4620SYBR FAST ROX Low500*20ul1800
KAPAKK4606SYBR® Fast（伯樂型）1 ml400
KAPAKK4607SYBR® Fast（伯樂型）5 ml1800
KAPAKK4608SYBR® Fast（伯樂型）10 ml3500
KAPAKK4609SYBR® Fast （Roche LC 480）1 ml400
KAPAKK4610SYBR® Fast（Roche LC 480）5 ml1800
KAPAKK4611SYBR® Fast（Roche LC 480）10 ml3500
SYBR 一步法QpcrKAPAKK46501-step qRT-PCR Kit(通用型）1ml1000
KAPAKK46511-step qRT-PCR Kit(通用型）5ml4500
KAPAKK46521-step qRT-PCR Kit(通用型）10ml8000
KAPAKK46601-step qRT-PCR Kit（ABI Prism）1ml1000
KAPAKK46611-step qRT-PCR Kit（ABI Prism）5ml4500
KAPAKK46621-step qRT-PCR Kit（ABI Prism）10ml8000
KAPAKK46701-step qRT-PCR Kit（伯樂型）1ml1000
KAPAKK46711-step qRT-PCR Kit（伯樂型）5ml4500
KAPAKK46721-step qRT-PCR Kit（伯樂型）10ml8000
KAPAKK46801-step qRT-PCR Kit（Roche LC 480）1ml1000
KAPAKK46811-step qRT-PCR Kit（Roche LC 480）5ml4500
KAPAKK46821-step qRT-PCR Kit（Roche LC 480）10ml8000
PROBE熒光定量KAPAKK4701PROBE FAST(通用型）1 ml400
KAPAKK4702PROBE FAST(通用型）5 ml1800
KAPAKK4703PROBE FAST(通用型）10 ml3500
KAPAKK4705PROBE FAST（ABI Prism）1 ml400
KAPAKK4706PROBE FAST（ABI Prism）5 ml1800
KAPAKK4707PROBE FAST（ABI Prism）10 ml3500
KAPAKK4709PROBE FAST（伯樂型）1 ml400
KAPAKK4710PROBE FAST（伯樂型）5 ml1800
KAPAKK4711PROBE FAST（伯樂型）10 ml3500
KAPAKK4714PROBE FAST（ABI Prism，定制50ml體系）50ml17000
KAPAKK4715PROBE FAST(通用型，定制50ml體系）50ml17000
KAPAKK4716PROBE FAST （ROX Low Mastermix）1ml400
KAPAKK4717PROBE FAST（ROX Low Mastermix）5 ml1800
KAPAKK4718PROBE FAST（ROX Low Mastermix）10 ml3500
KAPAKK4719PROBE FAST（ROX Low Mastermix）50 ml17000
KAPAKK4722PROBE FAST Univrsal qPCR Kit with dUTP500x20ul1800
PROBE Force qPCRKAPAKK4752PROBE FAST（1-step qRT-PCR Probe Universal）5 ml4500
KAPAKK4300通用型PROBE FORCE定量試劑1ml700
KAPAKK4301通用型PROBE FORCE定量試劑5ml3500
KAPAKK4302通用型PROBE FORCE定量試劑10ml6000
KAPAKK4303通用型PROBE FORCE定量試劑50ml26000
2G Robust酶及相關(guān)產(chǎn)品KAPAKK5023 2G Robust100 units400
KAPAKK5024 2G Robust250 units900
KAPAKK5004 2G Robust（含dNTPs）100units400
KAPAKK5005 2G Robust（含dNTPs）250 units900
KAPAkk5522 2G Robust Hot Start100units600
KAPAKK5515 2G Robust Hot Start250 units1400
KAPAKK5517 2G Robust Hot Start500 units2400
KAPAKK5525 2G Robust Hot Start2500units9000
KAPAKK5532 2G Robust Hot Start（含dNTPs）100 units700
KAPAKK5516 2G Robust Hot Start（含dNTPs）250 units1500
KAPAKK5518 2G Robust Hot Start（含dNTPs）500units2800
KAPAKK5701 2G Robust Hot Start Readymix100rxn700
KAPAKK5702 2G Robust Hot Start Readymix500 rxn2600
2G Fast酶及相關(guān)產(chǎn)品KAPAKK5523 2G Fast Hot Start100 units600
KAPAKK5503 2G Fast Hot Start250 units1300
KAPAKK5501 2G Fast Hot Start500 units2400
KAPAKK5519 2G Fast Hot Start2500 units7000
KAPAKK5530 2G Fast Hot Start（含dNTPs）100 units700
KAPAKK5502 2G Fast Hot Start（含dNTPs）250 units1500
KAPAKK5500 2G Fast Hot Start（含dNTPs）500 units2600
KAPAKK5513 2G Fast Hot Start（Buffer無Mg2+）250 units1400
KAPAKK5511 2G Fast Hot Start（Buffer無Mg2+）500 units2400
KAPAkk5512 2G Fast Hot Start（含dNTPs，Buffer無Mg2+）250 units1500
KAPAKK5510 2G Fast Hot Start（含dNTPs，Buffer無Mg2+）500 units2800
KAPAKK5603 2G Fast Hot Start ReadyMix100 rxn400
KAPAKK5601 2G Fast Hot Start ReadyMix500 rxn1400
KAPAKK5801 2G Fast Hot Start100 rxn500
KAPAKK5802 2G Fast Hot Start500 rxn1900

 

二代測序相關(guān)產(chǎn)品
　廠家貨號產(chǎn)品名稱規(guī)格目錄價(jià)
二代測序文庫制備KAPAKK8230KAPA LTP文庫構(gòu)建（含文庫擴(kuò)增）試劑盒－Illumina10個(gè)文庫2500
KAPAKK8231KAPA LTP文庫構(gòu)建（不含文庫擴(kuò)增）試劑盒－Illumina10個(gè)文庫2400
KAPAKK8232KAPA LTP文庫構(gòu)建（含文庫擴(kuò)增）試劑盒－Illumina 50個(gè)文庫11000
KAPAKK8233KAPA LTP文庫構(gòu)建（不含文庫擴(kuò)增）試劑盒－Illumina 50個(gè)文庫10500
KAPAKK8234KAPA HTP文庫構(gòu)建（含文庫擴(kuò)增）試劑盒－Illumina 96個(gè)文庫18000
KAPAKK8235KAPA HTP文庫構(gòu)建（不含文庫擴(kuò)增）試劑盒－Illumina96個(gè)文庫17000
KAPAKK8260KAPA TruSeq Adapter Kit, (Illumina, 20 μl each)144 rxn14000
KAPAKK2620KAPA Library Amplification Kit with Primers (50 x 50 µl reactions)50rxn1800
KAPAKK2621KAPA Library Amplification Kit with Primers (250 x 50 µl reactions)250rxn8000
KAPAKK2623KAPA Library Amplification Primer Kit (250 x 50 μl rxns)250rxn1500
KAPAKK8400KAPA Stranded RNA-Seq Library Preparation Kit (Illumina,24 reactions)24 rxn13000
KAPAKK8401KAPA Stranded RNA-Seq Library Preparation Kit (96 reactions)96 rxn40000
KAPAKK8420KAPA Stranded mRNA-Seq Library Preparation Kit (24 reactions)24 rxn15000
KAPAKK8421KAPA Stranded mRNA-Seq Library Preparation Kit (96 reactions)96 rxn47000
KAPAKK8483KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (24rxns)24rxn28000
KAPAKK8484KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (96rxns)96rxn98000
KAPAKK8500KAPA Hyper Prep Kit (8 reactions)8 rxn3100
KAPAKK8501KAPA Hyper Prep Kit, no amplification (8 reactions)8 rxn2970
KAPAKK8502KAPA Hyper Prep Kit (24 reactions)24 rxn8100
KAPAKK8503KAPA Hyper Prep Kit, no amplification (24 reactions)24 rxn7830
KAPAKK8504KAPA Hyper Prep Kit (96 reactions)96 rxn28100
KAPAKK8505KAPA Hyper Prep Kit, no amplification (96 reactions)96 rxn27000
KAPAKK8510KAPA Hyper Plus Kit (Illumina,8 rxns)8 rxn3500
KAPAKK8511KAPA Hyper Plus Kit, PCR-free (Illumina,8 rxns)8 rxn3370
KAPAKK8512KAPA Hyper Plus Kit (Illumina,24 rxns)24 rxn9300
KAPAKK8513KAPA Hyper Plus Kit, PCR-free (Illumina,24rxns)24 rxn9030
KAPAKK8514KAPA Hyper Plus Kit (Illumina,96 rxns)96 rxn32900
KAPAKK8515KAPA Hyper Plus Kit, PCR-free (Illumina,96 rxns)96 rxn31800
KAPAKK8300Ion Torrent  Library Preparation Kit (8 reactions)  8 rxn2520
KAPAKK8301Ion Torrent   Library Preparation Kit (48 reactions) 48 rxn13860
KAPAKK8310Ion Torrent Library Preparation Kit, no amplification (8 reactions)  8 rxn2280
KAPAKK8311Ion TorrentLibrary Preparation Kit, no amplification (48 reactions)48 rxn12420
KAPAKK8330Adapter Kit, non-Barcoded (8 reactions    Ion Torrent8 rxn1200
KAPAKK8331Adapter Kit, Barcodes 1 - 8 (48 reactions)   Ion Torrent48 rxn7500
KAPAKK8332Adapter Kit, Barcodes 9 - 16 (48 reactions)   Ion Torrent48 rxn7500
KAPAKK8333Adapter Kit, Barcodes 17 - 24 (48 reactions)   Ion Torrent48 rxn7500
二代測序文庫定量：標(biāo)準(zhǔn)品+引物預(yù)混物KAPAKK4800DNA Quant Kit   Roche 454 FLXEach6000
KAPAKK4801Primer Premix  Roche 454 FLXEach3000
KAPAKK4802DNA Quant Kit  Roche 454 TitaniumEach6000
KAPAKK4803Primer Premix Roche 454 TitaniumEach3000
KAPAKK4804DNA Quant Kit  Illumina Genome AnalyzerEach6000
KAPAKK4805Primer Premix  Illumina Genome AnalyzerEach3000
KAPAKK4806DNA Quant Kit  ABI SOLiDEach6000
KAPAKK4807Primer Premix  ABI SOLiDEach3000
KAPAKK4808DNA Quant Kit   Illumina GA Revised PrimersEach6000
KAPAKK4809Primer Premix  Illumina GA Revised PrimersEach3000
KAPAKK4812DNA Quant Kit - Ion TorrentEach6000
KAPAKK4813DNA Quantification Primer Premix -  Ion TorrentEach3000
KAPAKK4900Library Quantification DNA Standards (454 FLX/Lib-A)6 x 80 µl 4000
KAPAKK4902Library Quantification DNA Standards (SOLiD)6 x 80 µl 4000
KAPAKK4903Library Quantification DNA Standards (Illumina)6 x 80 µl 4000
KAPAKK4904Library Quantification DNA Standards (Ion Torrent)6 x 80 µl 4000
KAPAKK4923KK4923Library Quant Primers+KSF(IIIumina/Uni) IIIumina/Uni4000
KAPAKK4924kk4924 Library Quant primers+KSF(10mTorrent/uni)10mTorrent/uni5000
KAPAKK4820Library  Quant Kit，Roche 454 FLX-SYBR Fast UniversalEach7000
KAPAKK4821Library  Quant Kit，Roche 454 Titanium-SYBR Fast UniversalEach7000
KAPAKK4824Library  Quant Kit，Illumina GA revised primers -SYBR Fast UniversalEach7000
KAPAKK4823Library  Quant Kit，ABI SOLiD-SYBR Fast UniversalEach7000
KAPAKK4830Library  Quant Kit，Roche 454 FLX-SYBR Fast ABI PrismEach7000
KAPAKK4831Library  Quant Kit，Roche 454 Titanium-SYBR Fast ABI PrismEach7000
KAPAKK4835Library  Quant Kit，Illumina GA revised primers -SYBR Fast ABI PrismEach7000
KAPAKK4833Library  Quant Kit，ABI SOLiD-SYBR Fast ABI PrismEach7000
KAPAKK4840Library  Quant Kit，Roche 454 FLX-SYBR Fast Bio-Rad iCyclerEach7000
KAPAKK4841Library  Quant Kit，Roche 454 Titanium-SYBR Fast Bio-Rad iCyclerEach7000
KAPAKK4844Library  Quant Kit，Illumina GA revised primers-SYBR Fast Bio-Rad iCyclerEach7000
KAPAKK4843Library  Quant Kit，ABI SOLiD-SYBR Fast Bio-Rad iCyclerEach7000
KAPAKK4850Library  Quant Kit，Roche 454 FLX-SYBR Fast Roche Light Cycler480 Each7000
KAPAKK4851Library  Quant Kit，Roche 454 Titanium-SYBR Fast Roche Light Cycler 480Each7000
KAPAKK4854Library  Quant Kit，Illumina GA revised primers-SYBR Fast Roche Light Cycler480Each7000
KAPAKK4853Library  Quant Kit，ABI SOLiD-SYBR Fast Roche Light Cycler480Each7000
KAPAKK4834Library  Quant Kit，Illumina GA ABI Prism Custom Kit- SangerEach7000
KAPAKK4827Library  Quant Kit，Ion Torrent PGM universalEach7000
KAPAKK4838Library  Quant Kit，Ion Torrent PGM ABI PrismEach7000
KAPAKK4847Library  Quant Kit，Ion Torrent PGM  Bio-Rad iCyclerEach7000
KAPAKK4857Library  Quant Kit，Ion Torrent PGM  Roche Light Cycler480Each7000
KAPAKK4873KK4873Library Quant Kit (Illumina/ROX Low)500rxn7000
人類基因組定量Kit（文庫構(gòu)建之前用，主要檢測提取的DNA量）KAPAKK4960hg DNA Quant & QC kit with SYBR Universal qPCR MastermixEach8000
KAPAKK4961hg DNA Quant & QC kit with SYBR ABI Prism qPCR MastermixEach8000
KAPAKK4962hg DNA Quant & QC kit with SYBR Bio-Rad qPCR MastermixEach8000
KAPAKK4963hg DNA Quant & QC kit with SYBR Light Cycler 480 qPCR MastermixEach8000
KAPAKK4964hg DNA Quant & QC DNA Standards KitEach6000
KAPAKK4965hg DNA Quant & QC and 41 bp Primer KitEach1500
KAPAKK4966hg DNA Quant & QC and 129 bp Primer KitEach1500
KAPAKK4967hg DNA Quant & QC and 305 bp Primer KitEach1500
KAPAKK4969hg DNA Quant & QC Kit with ROX Low qPCR MastermixEach8000

 


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